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Interferon-gamma release assay performance in northeastern Brazil: influence of the IFNG + 874 A>T polymorphism BJID
Carneiro,Valdirene Leão; Bendicho,Maria Teresita; Santos,Rosalina Guedes; Casela,Marilda; Netto,Eduardo M.; Mota,Scarlet Torres Moraes; Pina,Iza Cristina Araújo; Nascimento,Roberto Meyer; Freire,Songeli Menezes; Barbosa,Theolis.
ABSTRACT Introduction Latent tuberculosis infection diagnosis based on the release of interferon-gamma in cultures of peripheral blood cells stimulated with Mycobacterium tuberculosis antigens has replaced the tuberculin skin test in many countries with low tuberculosis prevalence. The IFN-γ production can be influenced by genetic polymorphisms, of which the IFNG + 874 (rs62559044) locus is the most studied. We investigated the possible influence of the IFNG + 874 A/T polymorphism on interferon-gamma test performance. Methods Patients diagnosed with pulmonary tuberculosis (75), volunteers with positive tuberculin skin test (70) and healthy volunteers with negative tuberculin skin test and no history of contact with tuberculosis (57) were evaluated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tuberculosis infection; Genetic variation; Sensitivity.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000300202
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Is there genetic variation in seedless Argentinean grapefruit? Implications for crop production and conservation JBAG
Chacoff,N P; Souto,C P; Aizen,M.A.; Premoli,A.
Increasing crop genetic diversity has the potential to enhance pollination services, by contributing to the long-term stability of agroecosystems. Different species of the genus Citrus, including grapefruit, are cultivated worldwide. In Argentina, 30% of the total national yield is concentrated in the northwestern part of the country where new cultivars have arisen. We analyzed genetic variation and diversity within and among four plantations of Citrus paradisi for 11 isozyme loci. Although representing single cultivars clonally propagated, within plantations genetic variation was high. Observed heterozygosity ranged from 33 to 36.5%. The number of alleles per locus ranged from 2.4 to 2.6, and polymorphism from 72.7 to 81.8% and for the species in the area...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Citrus; Isozymes; Genetic variation; Upper Bermejo River Basin.
Ano: 2009 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332009000200001
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Isoenzymatic variability in wild potatoes PAB
Rocha,Beatriz Helena Gomes; Augustin,Eliane; Silva,João Baptista da; Viégas,Judith.
Two species of wild potato Solanum commersonii, subspecies commersonii and malmeanum, and S. chacoense, subspecies muelleri occur in southern Brazil. Their rusticity and easy adaptation to extreme climatic conditions make them valuable for breeding programs. The objective of this work was to analyze the isoenzymatic variability of 113 clones of wild potato subspecies. They were collected and maintained at Embrapa-Centro de Pesquisa Agropecuária de Clima Temperado, at Pelotas, RS, Brazil. Enzymes involved in energetic (group I) or in peripherical (group II) metabolism constituted the material used. Polyacrylamide horizontal gel electrophoresis was used to analyze peroxidase, aspartate transaminase, phosphoglucomutase and isocitrate dehydrogenase isoenzymes....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Peroxidases; Aminotransferases; Isomerases; Isocitrate dehydrogenase; Genetic variation.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2001000500008
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Limitada variabilidad genética de la quinta introducción en Cuba de Litopenaeus vannamei estimada con el uso de marcadores microsatélites OceanDocs
Artiles, A.; Rodríguez, I.; Pérez, A.; Pérez, L.; Espinosa, G..
Se estimó la variabilidad genética y el índice de parentesco entre lotes de camarón blanco del Pacífico, Litopenaeus vannamei, introducidos por quinta ocasión en Cuba, procedentes del Centro de mejora del camarón, de Estados Unidos (Shrimp Improving System: SIS), para su cultivo. La variabilidad genética se estimó mediante el genotipo de 33 muestras con cuatro loci microsatélites: M1, Pvan 1815, Pvan 0040 y Pvan 1758. El quinto lote de Litopenaeus vannamei tuvo los valores promedios de heterocigosidad esperada y observada, más bajos de todos los introduci- dos en Cuba: 0.37 y 0.27, respectivamente. Esto, unido a la poca cantidad de variantes alélicas para cada región microsatélite, indica una escasa variabilidad genética. Los valores del coeficiente...
Tipo: Journal Contribution Palavras-chave: Genetic variation.
Ano: 2011 URL: http://hdl.handle.net/1834/5346
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Local Seed Systems and Village-Level Determinants of Millet Crop Diversity in Marginal Environments of India AgEcon
Nagarajan, Latha; Smale, Melinda.
In the subsistence-oriented, semi-arid production systems of Andhra Pradesh and Karnataka, India, the environment is marginal for crop growth and often there is no substitute for millet crops. Across communities, farmers grow thirteen different combinations of pearl millet, sorghum, finger millet, little millet, and foxtail millet varieties, but individual farmers grow an average of only two to three millet varieties per season. The notion of the seed system includes all channels through which farmers acquire genetic materials, outside or in interaction with the commercial seed industry. Data are compiled through household surveys and interviews with traders and dealers in village and district markets. Based on the concept of the seed lot, several...
Tipo: Working or Discussion Paper Palavras-chave: Seed systems; Millet diversity; Variety change; Seed users; Crop diversity; Seed industry and trade; Genetic variation; Crop Production/Industries.
Ano: 2005 URL: http://purl.umn.edu/59229
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Low genetic variability in the fifth introduction of Litopenaeus vannamei in Cuba, as estimated with microsatellite markers OceanDocs
Artiles, A.; Rodríguez, I.; Pérez, A.; Pérez, L.; Espinosa, G..
The present work estimated the genetic variability and relatedness index of the fifth stock of Pacific white shrimp, Litopenaeus vannamei, imported into Cuba for farming purposes from the US Shrimp Improvement System (SIS). Ge- netic variability was estimated by genotyping 33 samples for four microsatellite loci: M1, Pvan 1815, Pvan 0040 and Pvan 1758. This stock had average expected and observed heterozygosities of 0.37 and 0.27 respectively; the lowest of all stocks previously introduced in Cuba. The above, together with the low amount of allelic variants detected for each microsatellite, was suggestive of low genetic variability. In addition, pairwise relatedness coefficients clustered around unity, indicating a high degree of consanguinity. Taken...
Tipo: Journal Contribution Palavras-chave: Genetic variation; Shrimp culture.
Ano: 2011 URL: http://hdl.handle.net/1834/5348
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Macrozooplankton diversity: how much do we really know? Naturalis
Pierrot-Bults, A.C.; Spoel, S. van der.
Pelagic species are widely distributed in a horizontal plane and have three-dimensional distributions, since the sea covers about 70% of the surface of the earth, and on average 4 km deep. For most macrozooplankton and micronekton groups there seems to be no great expectations of finding new taxa with the exception of gelatinous groups and of the deep sea, which is a relatively unknown especially in the benthopelagic zone close to the sea-bed. However, we do not know very much about variation, either morphological or genetical, within socalled species in different parts of their range. We think we are dealing with single very broadly distributed species, which, in reality, are species complexes consisting of several more or less cryptic species or...
Tipo: Article / Letter to the editor Palavras-chave: Pelagic macrozooplankton/micronekton diversity; Biogeographic patterns; Genetic variation; Morphological variation; Cryptic species; 42.79.
Ano: 2003 URL: http://www.repository.naturalis.nl/record/220316
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Mechanisms of adaptation to climate variability in West African pearl millet landraces – a preliminary assessment Open Agri
Haussmann, B.I.G..
Palavras-chave: Photoperiodicity; Planting date; Millets; Phenotypes; Drought stress; Genotypes; Genetic variation; Statistical methods; Replication; Breeds (animals).
Ano: 2007 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3312
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Melhoramento genético da pimenta-do-reino (Piper nigrum L.). Infoteca-e
POLTRONIERI, M. C.; ALBUQUERQUE, F. C. de; POLTRONIERI, L. S..
bitstream/item/57818/1/CPATU-PA179.pdf
Tipo: Séries anteriores (INFOTECA-E) Palavras-chave: Pimenta-do-reino; Resistência a doença; Brasil.; Melhoramento Genético Vegetal; Piper Nigrum; Variação Genética.; Amazonia; Disease resistance; Genetic variation; Plant breeding..
Ano: 1998 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/375214
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Morphological and growth responses to water stress of two sub-populations of Bromus pictus from soils with contrasting water availability RChHN
ROTUNDO,JOSÉ L; CIPRIOTTI,PABLO A; GUNDEL,PEDRO E.
We studied morphological and growth responses to drought of two sub-populations of Bromus pictus from communities with soils with different water availability from the Patagonian steppe. After a process of acclimatization common to both sub-populations, individuals from both sub-populations were subjected to three levels of water availability during 44 days. Independently of the water availability treatment, the sub-population from the community of high soil water availability showed higher aboveground relative growth rate (RGR) and higher leaf size (area and weight) compared to the sub-population from the community of low soil water availability. These results prove the expected evolution of higher growth rates and leaf sizes in resource rich...
Tipo: Journal article Palavras-chave: Arid lands; Drought; Ecotypes; Genetic variation; Soil depth.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2006000100006
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Morphological and molecular characterization of common bean landraces cultivated in the semi-arid Mexican high plateau Boletín de la Sociedad
Gill Langarica,Homar Rene; Rosales Serna,Rigoberto; Hernandez Delgado,Sanjuana; Mayek Perez,Netzahualcoyotl.
The objective of the current research was to characterize the common bean in agricultural fields planted with common bean landraces from the semi-arid Mexican high plateau using both morphological and amplified fragment length polymorphism (AFLPs) data. The morphological traits were discriminating and exhibited the clustering of 150 accessions based on the geographic origin and seed coat color. AFLP primer combinations exhibited a polymorphic range between 0.292 (E-AGG + M-ACT) and 0.375 (E-ACA + M-AGA). The frequency and distribution of the polymorphic fragments allowed the detection of a larger number of rare fragments in accessions 121 and 111 (Flor de Mayo and black common beans, respectively). The analysis of genetic relationships, analysis of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: AFLP; Genetic variation; Landraces; Phaseolus vulgaris L.
Ano: 2014 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-23722014000400010
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Origin of variability in maize Thai Agricultural
Dhawan, N.L.; Sprague, E.W..
1 ill.
Palavras-chave: Corn; Maize; Corn origin; Corn variability; Genetic variation; ข้าวโพด; แหล่งกำเนิด; ความแปรปรวนทางพันธุกรรม.
Ano: 1964 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/3742
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Pathogenicity mechanisms of prokaryotic cells: an evolutionary view BJID
Sousa,Cristina Paiva de.
The success of pathogenic microbes depends on their ability to colonize host tissues and to counter host defense mechanisms. Microorganisms can produce overwhelming infection because of their relatively short generation times, and because they have evolved powerful mechanisms for generating phenotypic diversity as an efficient strategy for adapting to rapidly responding immune system defenses and the broad range of polymorphisms characteristic of different host tissues. Bacterial evolution may not be a continuous process, but more of a succession of temporally spaced major events. These events cause a non-gradual sequence of adaptations to a given environment. The pathogenicity islands may be genetically unstable elements, and many of the genes coding for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pathogenicity; Prokaryotic evolution; Genetic variation; Bacterial virulence.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702003000100004
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Phylogenetic relationships among species of the subsection Dendrophlomis Bentham Electron. J. Biotechnol.
Yuzbasioglu,Ertugrul; Dadandi,Mehmet Yasar.
This study used randomly amplified polymorphic DNA markers to determine genetic relationships among species of the subsection Dendrophlomis. Twenty accessions of the eleven Phlomis taxa were evaluated to determine genetic variability using fourteen ten mer primers selected from a 125 random oligonucleotide set. These 14 selected primers generated 85 RAPD bands that ranged in size from 200 to 1200 base pairs. Of the total bands, 88% (75) were polymorphic among the samples. Genetic distances among accessions were computed to produce a dendrogram based on UPGMA. Genetic distances ranged from 0.133 (between P. amanica and P. monocephala) to 0.494 (between P. chimerae and P. lunariifolia). The UPGMA tree based on distances has two major groups. The first...
Tipo: Journal article Palavras-chave: Genetic diversity; Genetic relationships; Genetic variation; Phlomis species; RAPDs.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000400012
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Polymorphism of the grasshopper Rhammatocerus schistocercoides populations revealed by RAPD PAB
Silva,João Batista Tavares da; Tigano,Myrian Silvana; Magalhães,Bonifácio Peixoto; Cordeiro,Célia Maria Torres.
The objective of this work was to study the genetic variability of the grasshopper Rhammatocerus schistocercoides (Orthoptera: Acrididae) using RAPD analysis among individuals from three populations, one from Colombia and two from Brazil (Goiás and Mato Grosso States). Ninety scorable binary markers were obtained by fingerprinting with 11 oligonucleotide primers. Most of the polymorphism was attributed to 42 markers with variable frequency among the different populations. Although the existence of significant difference among populations (P<0.0001), most of the genetic variability was found within populations (87.7% of total variation). Pairwise distances between Colombian and Brazilian populations were 0.12 (P<0.0001) and 0.18 (P<0.0001) for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Insecta; Genetic marker; Genetic variation; Genetic distance.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2002001100020
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Prospects of breeding for micronutrients and β-carotene-dense sorghums Open Agri
Reddy, B.V.S.,( Belum V. S. Reddy ).
Palavras-chave: Carotenes; Grain; Organic acid salts; Agronomic characters; Heritability; Germplasm; Phenotypes; Genetic variation; Sorghum; Zinc.
Ano: 2005 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3256
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Quantificação da variabilidade genética entre matrizes de Coffea canephora das variedades botânicas Conilon e Robusta. Infoteca-e
ROCHA, R. B.; RAMALHO, A. R.; TEIXEIRA, A. L.; VIEIRA JUNIOR, J. R.; ESPINDULA, M. C.; Marcos Santana Moraes.
O objetivo desse trabalho foi quantificar a divergência genética entre 130 acessos clonais de Coffea canephora das variedades botânicas Conilon, Robusta e híbridos interespecíficos.
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E) Palavras-chave: Café Conilon; Híbrido interespecífico; Embrapa Rondônia; Ouro Preto d'Oeste (RO); Rondônia; Amazônia Ocidental; Western Amazon; Coffea Canephora; Café Robusta; Variação Genética; Campo Experimental; Genetic variation; Interspecific hybridization.
Ano: 2016 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1125208
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Random amplified polymorphic DNA profiles as a tool for the characterization of Brazilian keratitis isolates of the genus Acanthamoeba BJMBR
Alves,J.M.P.; Gusmão,C.X.; Teixeira,M.M.G.; Freitas,D.; Foronda,A.S.; Affonso,H.T..
The genus Acanthamoeba comprises free-living amebae identified as opportunistic pathogens of humans and other animal species. Morphological, biochemical and molecular approaches have shown wide genetic diversity within the genus. In an attempt to determine the genetic relatedness among isolates of Acanthamoeba we analyzed randomly amplified polymorphic DNA (RAPD) profiles of 11 Brazilian isolates from cases of human keratitis and 8 American type culture collection (ATCC) reference strains. We found that ATCC strains belonging to the same species present polymorphic RAPD profiles whereas strains of different species show very similar profiles. Although most Brazilian isolates could not be assigned with certainty to any of the reference species, they could...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acanthamoeba; Keratitis; RAPD typing; Genetic variation.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000100003
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Residual effects of successive exposure of soybean Bradyrhizobium strains to aluminium on solid defined medium PAB
Campo,Rubens José; Wood,Martin.
The aim of these studies was to investigate whether residual toxic effects of exposing soybean root nodule bacteria to Al in a solid defined media (SDM) alter tolerance to Al, survival, sensitivity to antibiotics, N2 fixation effectiveness and genetic diversity of Bradyrhizobium strains. After being exposed four times to Al, strains showed variation in Al tolerance but there was no evidence of change in their original Al tolerance, sensitivity to the antibiotics or genetic diversity. Exposure of Bradyrhizobium strains to SDM plus Al did not alter biological N2 fixation effectiveness of five strains. Strain SEMIA 587 showed a reduction in its N2 fixation effectiveness but it seems that it was just a superficial toxic effect because one single passage...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nitrogen fixation; Antibiotics; Resistance to chemicals; Genetic variation.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2001001100011
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Seed yield of simple and trí-línear corn (Zea mays L.) hybrids for the mexican humid tropics Acta Agron. (Palmira)
Macías,Mauro Sierra; Rodríguez Montalvo,Flavio A.; Espinosa Calderón B,Alejandro; Tadeo Robledo,Margarita; Gómez Montiel,Noel Orlando; Vázquez Carrillo,María Griselda; Ugalde Acosta,Francisco Javier; Freyre,Sabel Barrón; Arellano,José Luis Del Rosario; Leyva Ovalle,Otto Raúl; Serna Lagunes,Ricardo; Meza,Pablo Andrés.
Abstract The objective of the work was to evalúate the agronomic performance and seed yield of tropical corn (Zea mays L.) of single and tri-linear crosses. In the spring-summer of 2015 and 2016, two essays were established in the states of Veracruz and Tabasco, Mexico. In the first essay, a group of 12 single crosses was evaluated, and in the second, a group of 18 tri-linear crosses was evaluated. In both essays, the treatments were distributed under a completely randomized block design with three replicates in 5 m long, two-row plots and 0.8 m between rows, with an initial population density of 62.500 plants ha-1. Within the simple cross trial, hybrids HE-3W, COMERCIAL 1, LT-164xLT-165, and HE-1W presented seed yields above the general mean (3384 kg...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agronomic performance; Genetic variation; Heterosis; Hybridization; Productivity.
Ano: 2021 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122021000200180
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